Journal: Pharmaceuticals

Article Title: Antibacterial and Antimycotic Activity of Epilobium angustifolium L. Extracts: A Review

doi: 10.3390/ph16101419

Figure Lengend Snippet: Antibacterial activity of Epilobium angustifolium extracts.

Article Snippet: , pyogenes , ATCC 12345 , , 325 μg/mL [ ] .

Techniques: Activity Assay, Inhibition, Isolation

Journal: Biomedicines

Article Title: Assessment of the Relationship between Clinical Manifestation and Pathogenic Potential of Streptococcus pyogenes Strains-Distribution of Genes and Genotypes of Toxins

doi: 10.3390/biomedicines10040799

Figure Lengend Snippet: The percentages of genes presence and genotype distribution among the examined S. pyogenes strains ( n = 147); (+)–gene presence confirmed, (−)–lack of a particular gene.

Article Snippet: The DNA isolated from S. pyogenes : DSMZ 20565 strain (for the speA and speJ genes), DSMZ 25932 strain (for the speB , speC , and speH genes ) and DSMZ 11728 strain (for speK gene) served as PCR positive control.

Techniques:

Journal: Biomedicines

Article Title: Assessment of the Relationship between Clinical Manifestation and Pathogenic Potential of Streptococcus pyogenes Strains-Distribution of Genes and Genotypes of Toxins

doi: 10.3390/biomedicines10040799

Figure Lengend Snippet: Occurrence of the virulence factor genes with respect to the origin of S. pyogenes strains from selected types of clinical material.

Article Snippet: The DNA isolated from S. pyogenes : DSMZ 20565 strain (for the speA and speJ genes), DSMZ 25932 strain (for the speB , speC , and speH genes ) and DSMZ 11728 strain (for speK gene) served as PCR positive control.

Techniques:

Journal: Biomedicines

Article Title: Assessment of the Relationship between Clinical Manifestation and Pathogenic Potential of Streptococcus pyogenes Strains-Distribution of Genes and Genotypes of Toxins

doi: 10.3390/biomedicines10040799

Figure Lengend Snippet: The distribution of genotypes A–F with respect to origin of S. pyogenes strains from selected types of clinical material.

Article Snippet: The DNA isolated from S. pyogenes : DSMZ 20565 strain (for the speA and speJ genes), DSMZ 25932 strain (for the speB , speC , and speH genes ) and DSMZ 11728 strain (for speK gene) served as PCR positive control.

Techniques:

Journal: Medical Microbiology and Immunology

Article Title: LDL acts as an opsonin enhancing the phagocytosis of group A Streptococcus by monocyte and whole human blood

doi: 10.1007/s00430-015-0436-8

Figure Lengend Snippet: Characteristics of GAS strains

Article Snippet: Three GAS strains M6 (ATCC BAA946), M28 (ATCC BAA1064), and M41 (ATCC 12373, AM41) were purchased from American Tissue Culture Collection, and one strain M41 (CMCC 32198, CM41) was obtained from China Medical Culture Collection Center, respectively.

Techniques: Expressing, Binding Assay

Journal: Medical Microbiology and Immunology

Article Title: LDL acts as an opsonin enhancing the phagocytosis of group A Streptococcus by monocyte and whole human blood

doi: 10.1007/s00430-015-0436-8

Figure Lengend Snippet: LDL-mediated phagocytosis of four GAS strains. After 30-min incubation of U937 cells with FITC-labeled GAS DAPI was added and co-cultivated for 10 min; 5 ml of sample was plated onto slides, and GAS ( green ) and DAPI-stained U937 cell nuclei ( blue ) were imaged, respectively, at ×200 magnification on fluorescent microscope. a1 , b1 , c1 and d1 refer to control group, including AM41, CM41, M28 and M6-type GAS without LDL, whereas a2 , b2 , c2 and d2 stand for the treatments with LDL. The colony-forming units (cfu) of GAS in the mixture were determined by plating diluted samples onto THY agar plates after 15-, 30- and 60-min co-culture of FITC-GAS and U937 cells in the presence or absence of LDL. The phagocytosis rates were expressed as 100 % × (cfu of addition − cfu after co-culture)/cfu of addition. The red lines in a3 , b3 , c3 and d3 refer to the phagocytosis rates calculated upon cfu. Bacterial fluorescence intensity was monitored at 15, 30 and 60 min of incubation, and 200 µl of sample was pipetted into the 96-well microplate and measured at λ = 495 nm (excitation) and λ = 519 nm (emission) using a fluorescence plate reader. The phagocytosis rates were expressed as 100 % × (initial absorbance value − absorbance value after co-culture)/initial absorbance value. The green lines in a3 , b3 , c3 and d3 refer to the phagocytosis rates calculated upon fluorescence (fluor)

Article Snippet: Three GAS strains M6 (ATCC BAA946), M28 (ATCC BAA1064), and M41 (ATCC 12373, AM41) were purchased from American Tissue Culture Collection, and one strain M41 (CMCC 32198, CM41) was obtained from China Medical Culture Collection Center, respectively.

Techniques: Incubation, Labeling, Staining, Microscopy, Control, Co-Culture Assay, Fluorescence